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Run order of samples was randomized and in lieu of internal standards, technical replicates were injected every 8 samples to assess quality control and instrument performance

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The change in pH of TSB and 1 PBS at different GSH concentrations was determined using a pH meter (Mettler-Toledo GmbH, Greifensee, Switzerland) calibrated with pH standards of 4.0 and 7.0 and the pH values of TSB and 1 PBS as a function of GSH concentration reported in Table 2

Diabetes (1995) 44:86370

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