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Full protocol with comprehensive search strategy, filters, keywords, and combinations is available in Supplementary material 1

Basal cell cycle distribution has been implicated in the radio response, with cells in the S phase and G0/G1 phases being more radioresistant, and cells in the G2/M phase being the most sensitive to radiation exposure ( Metformin treatment significantly reduced the proportion of radiosensitive HCT116 cells in the G0/G1 phase at 48 h post treatment, when compared to vehicle control ( Figure 2A )

This dual agonist approach was a breakthrough when it first hit the market, outperforming older single-receptor drugs like semaglutide in head-to-head trials

At 48 h post-transfection, cells were harvested in ice-cold PBS using Trypsin-EDTA (Lonza, Switzerland), centrifuged at 1000 g for 3 min, and washed with ice-cold PBS

Can vary with work or activities that require regular active mouth movements Repeated treatments are necessary to maintain results