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Therefore, the key step in the formation of pannus is angiogenesis, which provides a resupply for proliferating and migrating synovial cells and aggravates cartilage destruction and erosion (182)

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Photomicrographs were captured every 2 h, and real-time confluence measurements were performed using the IncuCyte Base Analysis Software (Sartorius, Germany)

Shaking adds air, creates foam, and can fracture peptide bonds