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Each reaction contains 250 nM TrxR1, 300 M Nicotinamide adenine dinucleotide phosphate (NADPH) (N5130-25 MG, Sigma) and 5 mM DTNB in buffer containing 100 mM potassium phosphate, 1 mM Ethylenediaminetetraacetic acid (EDTA) (E4378-25G, Sigma), pH 7.0

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HERA 48 was performed by seeding HMEC-1-FcRn cells (1.5 10 5 cells/well) to a Costar 48-well TC-Treated Multiple Well Plate (Corning) in complete culture medium (250 L/well) for approximately 2024 h until confluent