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Viral DNA was extracted from blood samples of both captive and free-ranging khulan using a commercially available kit (QIAamp DNA Blood Mini Kit, Giagen) and from nasal swabs using the Invisorb Spin Virus DNA Mini Kit, Stratec, according to the manufactures instructions

Among these, 15 metabolites, mainly phenolic acids and flavonoids, were significantly enriched in the SM group

Reversed phase gives the most information per run, but cannot see aggregates, size-exclusion sees aggregates, but not sequence variants, and mass spectrometry confirms identity but depends on a clean upstream separation

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Thus, when ascending from C in half steps, we get C, C#, D, D#, E, F, F#, G, G#, A, A#, B, and back to C

save intense exercise for week 5 onward when energy levels stabilize