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After a second purification with AMPure XP beads, the final amplicon was quantified using the Qubit dsDNA Assay Kit (Thermo Fisher Scientific, USA), and the concentrations were then adjusted for sequencing

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The side chain of Leu 123 is flipped toward Arg 121 , which again is flipped toward Pro 119 , thereby closing an otherwise water-accessible cavity observed in the exendin-4(939)-bound structure (Fig

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